Industrial Sites:

Industrial plants as cooling towers, composting plants, waste sorting plants, green waste collection centers or wastewater treatment plants could involve the release of a wide range of chemical and biological pollutants with potential harmful impact on workers’ health (respiratory issues) and people living in neighbouring residential areas.

Many studies are reported high concentrations of fungi, bacteria, endotoxins and (1_3) beta-glucans in different types of waste management plants.

Site_industriel2

The classical method used for air sampling relies on impaction on agar dishes and the culture of the micro-organisms collected. In highly contaminated environments as facilities managing liquid or solid waste, the use of conventional methods is not appropriated for many reasons:

  • low volume of air collected,
  • Petri dish saturated,
  • bacteria or fungi concentration underestimated,
  • predominant rapidly growing bacteria or fungi may mask the presence of other species…

The results will be thus difficult/impossible to interpret and not always representative of the controlled environment. Further, culture methods do not allow for differentiation between toxigenic and non toxigenic strains, whether viable or non viable fungi particles.

A number of studies suggested that both molecular and conventional methods need to be employed in the identification and quantification of microorganisms to overcome some of the limitations in conventional methodology. The Coriolis µ is used in many studies if such contaminated sites: rapid microbiological methods (as quantitative RT-PCR assays, solid phase cytometry analysis, DAPI labeling) or decimal dilution before spreading in the agar media can be performed from the Coriolis liquid sample.